Project information
Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine
- Project Identification
- LC06035
- Project Period
- 3/2006 - 12/2011
- Investor / Pogramme / Project type
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Ministry of Education, Youth and Sports of the CR
- Basic Research Center
- MU Faculty or unit
- Faculty of Science
- Keywords
- nukleové kyseliny, proteiny, karcinogeny, léčiva, poškození DNA, hybridizace DNA, interakce DNA-protein, nádorové supresory, amyloidní proteiny, mutantní proteiny, denaturace, agregace, elektrochemie, separace, kapilární elektroforéza, hmotová spektromet
- Cooperating Organization
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Institute of Biophysics
Charles University
University of Pardubice
Masaryk Memorial Cancer Institute Brno
After completion of the human genome project, there is demand for simple and inexpensive methods enabling analysis of details of individuals' genomes. The contemporary world science is oriented towards utilization of electrochemical DNA detection in thisanalysis. DNA electroactivity was discovered at the Institute of Biophysics in Brno. Laboratories of this Institute belong to the world elite in this field for more than four decades. In the partner laboratories, a considerable progress has been attainedin the research of electrochemical DNA sensors as well as in analysis of proteins and other substances in recent years. This project is based on the existing results and it is oriented towards advanced studies of the properties of biomacromolecules andt heir interactions with surfaces, especially electrodes, and towards research of intermolecular interactions of DNA and/or proteins important in severe disorders (cancer, Parkinson's or Alzheimer's disease). On the basis of electrochemistry, miniaturiza
Publications
Total number of publications: 228
2008
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Biomarker discovery in low grade breast cancer using isobaric stable isotope tags and two-dimensional liquid chromatography-tandem mass spectrometry (iTRAQ-2DLC-MS/MS) based quantitative proteomic analysis
Year: 2008, type: Conference abstract
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CE study of neuroprotective humanin peptide and its derivatives: Interactions with phosphate, sulphate, alkylsulphonates and sulphated-beta-CD
Electrophoresis, year: 2008, volume: 29, edition: 3, DOI
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Combined 3-in-1 photometric, fluorimetric and contactless conductometric on-capillary detector for capillary separations: Applicability for simultaneous detection in CE
Year: 2008, type: Conference abstract
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Combined detector for on capillary photometric, fluorimetric and contactless conductometric detection in microcolumn separations
Year: 2008, type: Conference abstract
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Combining photometry, fluorimetry and contactless conductometry in on-capillary detection for capillary electrophoresis and nano-liquid chromatography
Year: 2008, type: Conference abstract
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DISSOCIATION KINETICS STUDY OF Ce(III) COMPLEXES WITH H8dotp (H8dotp = 1,4,7,10-TETRAAZACYCLODODECANE-1,4,7,10-TETRAKIS(METHYLPHOSPHONIC ACID))
Journal of Alloys and Compounds, year: 2008, volume: 451, edition: 1-2
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Electrochemical Study of Copper-Aminopurines Complexes at Carbon Electrodes
6th International Copper Meeting. Book of Abstracts, year: 2008
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Elimination Voltammetry of Oligonucleotides
Electrochemistry of Nucleic Acids and Proteins. Book of Abstracts, year: 2008
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Chemical and biological evaluation of 153Sm and 166Ho complexes of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetrakis(methylphosphonic acid monoethylester) (H4dotpOEt)
Journal of Inorganic Biochemistry, year: 2008, volume: 102, edition: 7
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Identification of alphaB-crystallin, a biomarker of renal cell carcinoma by SELDI-TOF MS
The International Journal of Biological Markers, year: 2008, volume: 23, edition: 1