Project information
Proteins in metabolism and interaction of organisms with the environment
- Project Identification
- MSM0021622413
- Project Period
- 1/2005 - 12/2011
- Investor / Pogramme / Project type
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Ministry of Education, Youth and Sports of the CR
- Research Intents
- MU Faculty or unit
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Faculty of Science
- prof. RNDr. Jaroslav Koča, DrSc.
- Other MU Faculty/Unit
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Central European Institute of Technology
- Keywords
- proteins, structure function relationships, protein complexes, proteome, metabolome
The proposal is the follow up of the previous, in our view successful, long-term research plan "Structure-Function Relationships of Biomolecules and their Role in Metabolism" studied in 1998-2004, and partly the project "Laboratory of Structure and Dynamics of Biomolecules" in the framework of program "250" studied in 1996-2000. The results of these projects were published in tens of scientific articles communicated in renowned international journals with high impact factors. The key aim was identification and characterization of low molecular weight substances involved in metabolism of bacteria and fungi with the special attention devoted to biochemical activities and the structure.
This new proposal will deal with other proteins from various organisms, for example animal proteins binding pheromones or proteins interacting with sacharides. The function and regulation of enzymes will be studied by novel methods suitable for monitoring of in vivo activity via monitoring of intracellular metabolites. We will continue to use structural methods. Keywords of the long-term research plan are protein, its function, structure, structure-function relationships and complexes of proteins. More general keywords are proteome and metabolome. The project will focus on such hot topics of current life sciences like protein/DNA interactions, synthesis of proteins on ribosome or applications of photo-labile groups for directed geometrical changes of protein-substrate complexes and regulation of enzymatic reactions.
Publications
Total number of publications: 835
2011
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Biology and Bioinformatics
Year: 2011, type: Appeared in Conference without Proceedings
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Bioluminescent assay as a tool for monitoring metabolic activity of acidophilic bacteria
Year: 2011, type: Conference abstract
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Burkholderia cenocepacia BC2L-C Is a Super Lectin with Dual Specificity and Proinflammatory Activity
PLoS Pathogens, year: 2011, volume: 7, edition: 9, DOI
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Carbohydrate CH/pi interactions – Theoretical investigation of the favourable regions in the carbohydrate surroundings
Year: 2011, type: Conference abstract
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Combination of Transverse and Longitudinal Diffusion as a Generic Approach for Reactants Mixing inside the Capillary
Year: 2011, type: Conference abstract
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Comparison of protein structural motifs – challenges and algorithms. Improved approach and case studies
Year: 2011, type: Conference abstract
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Comparison of Two Electrophoretic Methods Documented by Separation of Dextromethorphan and its Metabolites
Year: 2011, type: Conference abstract
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Complementation of 3D structure of delta subunit of RNA polymerase from Bacillus subtilis with description of internal motions in terms of reduced spectral density mapping
MATERIALS STRUCTURE, year: 2011, volume: 18, edition: 1
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Computational study of the dimethylphosphate hydrolysis as the reference system for the understanding of the restriction endonucleases mechanism
Year: 2011, type: Conference abstract
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Crystallization and Preliminary X-ray Diffraction Analysis of the Wild-Type Haloalkane Dehalogenase DhaA and its Variant DhaA13 Complexed with Different Ligands
Acta Crystallographica F, year: 2011, volume: 67, edition: 67, DOI