Project information
Proteins in metabolism and interaction of organisms with the environment
- Project Identification
- MSM0021622413
- Project Period
- 1/2005 - 12/2011
- Investor / Pogramme / Project type
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Ministry of Education, Youth and Sports of the CR
- Research Intents
- MU Faculty or unit
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Faculty of Science
- prof. RNDr. Jaroslav Koča, DrSc.
- Other MU Faculty/Unit
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Central European Institute of Technology
- Keywords
- proteins, structure function relationships, protein complexes, proteome, metabolome
The proposal is the follow up of the previous, in our view successful, long-term research plan "Structure-Function Relationships of Biomolecules and their Role in Metabolism" studied in 1998-2004, and partly the project "Laboratory of Structure and Dynamics of Biomolecules" in the framework of program "250" studied in 1996-2000. The results of these projects were published in tens of scientific articles communicated in renowned international journals with high impact factors. The key aim was identification and characterization of low molecular weight substances involved in metabolism of bacteria and fungi with the special attention devoted to biochemical activities and the structure.
This new proposal will deal with other proteins from various organisms, for example animal proteins binding pheromones or proteins interacting with sacharides. The function and regulation of enzymes will be studied by novel methods suitable for monitoring of in vivo activity via monitoring of intracellular metabolites. We will continue to use structural methods. Keywords of the long-term research plan are protein, its function, structure, structure-function relationships and complexes of proteins. More general keywords are proteome and metabolome. The project will focus on such hot topics of current life sciences like protein/DNA interactions, synthesis of proteins on ribosome or applications of photo-labile groups for directed geometrical changes of protein-substrate complexes and regulation of enzymatic reactions.
Publications
Total number of publications: 835
2011
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Crystallization and structure determination of delta-subunit of RNApolymerase
Year: 2011, type: Conference abstract
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Development of a Crystallization Protocol for the DbeA1 Variant of Novel Haloalkane Dehalogenase from Bradyrhizobium elkani USDA94
Crystal Growth & Design, year: 2011, volume: 11, edition: 2, DOI
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Development of radiometric enzyme assays: Studies of the interaction of fluoxetine with thyroid hormones metabolism
Year: 2011, type: Conference abstract
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Directed evolution of lectins from pathogenic bacteria
Year: 2011, type: Conference abstract
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Dispersion interactions of carbohydrates with condensate aromatic moieties: Theoretical study on the CH–pi interaction additive properties
Physical Chemistry Chemical Physics, year: 2011, volume: 13, edition: 31, DOI
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Docking study of matrix metalloproteinase inhibitors
Year: 2011, type: Conference abstract
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Effect of Mg2+ Binding on the Receiver Domain of the CKI1 Sensory Histidine Kinase from Arabidopsis thaliana
Year: 2011
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Elicitine-membrane interaction is driven by a positive charge on the protein surface: Role of Lys13 residue in lipids loading and resistance induction
Plant Physiology and Biochemistry, year: 2011, volume: 49, edition: 3, DOI
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Engineering Robust and Efficient Protein Catalysts
Year: 2011, type:
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Fast and Accurate calculation of Lectin/Saccharide Binding Free Energy
Year: 2011, type: Conference abstract