Project information
Proteins in metabolism and interaction of organisms with the environment
- Project Identification
- MSM0021622413
- Project Period
- 1/2005 - 12/2011
- Investor / Pogramme / Project type
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Ministry of Education, Youth and Sports of the CR
- Research Intents
- MU Faculty or unit
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Faculty of Science
- prof. RNDr. Jaroslav Koča, DrSc.
- Other MU Faculty/Unit
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Central European Institute of Technology
- Keywords
- proteins, structure function relationships, protein complexes, proteome, metabolome
The proposal is the follow up of the previous, in our view successful, long-term research plan "Structure-Function Relationships of Biomolecules and their Role in Metabolism" studied in 1998-2004, and partly the project "Laboratory of Structure and Dynamics of Biomolecules" in the framework of program "250" studied in 1996-2000. The results of these projects were published in tens of scientific articles communicated in renowned international journals with high impact factors. The key aim was identification and characterization of low molecular weight substances involved in metabolism of bacteria and fungi with the special attention devoted to biochemical activities and the structure.
This new proposal will deal with other proteins from various organisms, for example animal proteins binding pheromones or proteins interacting with sacharides. The function and regulation of enzymes will be studied by novel methods suitable for monitoring of in vivo activity via monitoring of intracellular metabolites. We will continue to use structural methods. Keywords of the long-term research plan are protein, its function, structure, structure-function relationships and complexes of proteins. More general keywords are proteome and metabolome. The project will focus on such hot topics of current life sciences like protein/DNA interactions, synthesis of proteins on ribosome or applications of photo-labile groups for directed geometrical changes of protein-substrate complexes and regulation of enzymatic reactions.
Publications
Total number of publications: 836
2009
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Functional significance of the Rad51-Srs2 complex in Rad51 presynaptic filament disruption.
Nucleic Acids Research, year: 2009, volume: 20, edition: 37
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Genetická analýza tří koster z neolitické těžní šachty v Krumlovském lese
15. Kvartér 2009, year: 2009
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Heterologous expression and molecular characterization of the NAD(P)H:acceptor oxidoreductase (FerB) of Paracoccus denitrificans
Protein Expression and Purification, year: 2009, volume: 68, edition: 2
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Changes of binding specificity and thermodynamical parameters of lectin from Chromobacterium violaceum - carbohydrate binding site mutagenesis in the position 97
XIII. Setkání biochemiků a molekulárních biologů, year: 2009
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Characterization of Haloalkane Dehalogenases DrbA and DmbC: Representatives of Novel Subfamily.
Applied and Environmental Microbilogy, year: 2009, volume: 75, edition: 15
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Characterization of the quinone reductase activity of the ferric reductase B protein from Paracoccus denitrificans
Archives of biochemistry and biophysics, year: 2009, volume: 483, edition: 1
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Charge Localization on Imidazolium Ring upon Complexation with Cucurbit[6]uril
Year: 2009, type: Conference abstract
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Implementation and Testing of Multiple Walkers Approach Based Free Energy Calculations in the Grid Environment
GCCP Proceedings, year: 2009
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Implementation of Parallel Analyzes of Molecular Dynamics Simulation Trajectories
Year: 2009, type: Conference abstract
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Importance of oligomerisation on Pseudomonas aeruginosa Lectin-II binding affinity. In silico and in vitro mutagenesis.
Journal of Molecular Modeling, year: 2009, volume: 15, edition: 6