Project information
Apoptosis-inducing factor (AIF): its translocation from mitochondria and its action on nuclear chromatin
- Project Identification
- GP204/03/D031
- Project Period
- 9/2003 - 8/2006
- Investor / Pogramme / Project type
-
Czech Science Foundation
- Postdoctoral projects
- MU Faculty or unit
- Faculty of Informatics
- Keywords
- Apoptosis, AIF, Chromatin, Chromosomal territories, Cytochrome c, Nuclear architecture
Apoptosis is an important physiological process, that represents a natural way of the cell death. During the permeabilization of mitochondrial
membrane in apoptosis, the outer mitochondrial membrane becomes soluble to proteins including cytochrome c and apoptosis-inducing factor, AIF. After a stimulation of apoptosis, AIF ranslocates into the cytoplasm and then into the cell nucleus, where it causes the chromatine condensation and large (-50 kbp) DNA fragmentation. The consequences of AIF action on the structure of cell nucleus and on the structure of chromosomal territories are still unknown. Our innovative experiments should lead to new results further defining AIF action in cell nucleus. We will also study the mechanism of translocation of AIF and cytochrome c from mitochondria and connection of this mechanism with changes in mitochondrial membrane potential during apoptosis.
Results
Publications
Total number of publications: 8
2007
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Bioinformatic and image analyses of cellular localization of apoptotic proteins endonuclease G, AIF, and AMID
Year: 2007, type:
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Bioinformatic and image analyses of the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID during apoptosis in human cells
Apoptosis, year: 2007, volume: 12, edition: 7
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Fluorescent microscopy of living cells
Year: 2007, type: Conference abstract
2006
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Analýza lokalizace a interakce fluorescenčně značených molekul v živých i fixovaných lidských buňkách.
Year: 2006, type: Conference abstract
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Single-cell image analysis of cellular localization and colocalization of endonuclease G, AIF and AMID in living cells
Year: 2006, type: Conference abstract
2005
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Imaging of mitochondrial apoptogenic proteins released during apoptosis in living and fixed cells.
Year: 2005, type: Conference abstract
2004
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Automated in vivo imaging of cell interior using fluorescent proteins
Year: 2004, type: Conference abstract
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In vivo imaging of cell interior using automated high-resolution cytometry
Biophysics of the Genome, year: 2004