Bioinformatic and image analyses of the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID during apoptosis in human cells

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Authors

VAŘECHA Miroslav AMRICHOVÁ Jana ZIMMERMANN Michal ULMAN Vladimír LUKÁŠOVÁ Emilie KOZUBEK Michal

Year of publication 2007
Type Article in Periodical
Magazine / Source Apoptosis
MU Faculty or unit

Faculty of Informatics

Citation
Field Genetics and molecular biology
Keywords apoptosis-inducing factor; endonuclease G; AIF-homologous mitochondrion-associated inducer of death; living cells; colocalization image analysis; analysis of amino acid sequence
Description We studied the cellular localization of the apoptotic proteins endonuclease G, AIF, and AMID in silico using three prediction tools and in living cells using both single-cell colocalization image analysis and nuclear translocation analysis. We confirmed the mitochondrial localization of endonuclease G and AIF by prediction analysis and by single-cell colocalization image analysis. We found the AMID protein to be cytoplasmic, most probably incorporated into the cytoplasmic side of the membranes of various organelles. The highest concentration of AMID was observed associated with the Golgi. Colocalization of AMID with lysosomes was also indirectly confirmed by analysis of AMID-rich vesicle velocity using manual tracking analysis. Bioinformatic analysis also detected nuclear localization signals in endonuclease G and AIF, but not in AMID. A novel analysis of time-lapse fluorescence image data during staurosporine-induced apoptosis revealed nuclear translocation only for endonuclease G and AIF.
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