Automated in vivo imaging of cell interior using fluorescent proteins
| Authors | |
|---|---|
| Year of publication | 2004 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Techniques with fluorescent proteins have become very valuable tools for the study of cellular processes in living cells. These proteins enable the non-invasive quantitative visualization in vivo as molecular tags on natively non-fluorescent molecules of interest. Fluorescent proteins are welcome innovation in the field of cell biology, because in vivo experiments bring new interesting results and different point of view than in vitro and in situ experiments. This presentation will address automated 2D/3D high-resolution confocal image acquisition and analysis of cells stained with fluorescent proteins. While the approaches to automated in situ imaging of cell interior have been previously described by our group (Cytometry 45, 1-12, 2001), this contribution will concentrate on the modifications (both hardware and software ones) of our high-resolution image cytometers made for the purpose of automated in vivo imaging. |
| Related projects: |