Stanovení koncentrace celkové bílkoviny v moči s pozitivní Bence Jonesovou bílkovinou
| Title in English | Determination of total protein concentration in urine with positive Bence Jones protein |
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| Authors | |
| Year of publication | 2009 |
| Type | Article in Periodical |
| Magazine / Source | Klinická biochemie a metabolismus |
| MU Faculty or unit | |
| Citation | |
| Field | Oncology and hematology |
| Keywords | Bence Jones protein;proteinuria; immunoglobulins; free light chains;monoclonal gammopathies |
| Description | We have carried out an external quality control assessment with the participation of 79 clinical biochemistry laboratories from the Czech Republic and Slovakia. The laboratories received a reference urine sample obtained from a patient with multiple myeloma and lambda FLC proteinuria and were asked to type the paraprotein using immunofi xation and to measure total urinary protein using their established method, most commonly turbidimetry, pyrogallol red assay, and biuret assay. There was a very wide inter-laboratory variability in the protein concentration readouts with up to three-fold difference in some cases. High-resolution two-dimensional electrophoresis showed that a high proportion of the urinary paraprotein was composed of lambda light chain fragments with molecular weight of 12 kDa. Our results highlight the challenges of reliable and reproducible measurement of urinary protein concentration in the presence of urinary FLCs. |