Project information
Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine
- Project Identification
- LC06035
- Project Period
- 3/2006 - 12/2011
- Investor / Pogramme / Project type
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Ministry of Education, Youth and Sports of the CR
- Basic Research Center
- MU Faculty or unit
- Faculty of Science
- Keywords
- nukleové kyseliny, proteiny, karcinogeny, léčiva, poškození DNA, hybridizace DNA, interakce DNA-protein, nádorové supresory, amyloidní proteiny, mutantní proteiny, denaturace, agregace, elektrochemie, separace, kapilární elektroforéza, hmotová spektromet
- Cooperating Organization
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Institute of Biophysics
Charles University
University of Pardubice
Masaryk Memorial Cancer Institute Brno
After completion of the human genome project, there is demand for simple and inexpensive methods enabling analysis of details of individuals' genomes. The contemporary world science is oriented towards utilization of electrochemical DNA detection in thisanalysis. DNA electroactivity was discovered at the Institute of Biophysics in Brno. Laboratories of this Institute belong to the world elite in this field for more than four decades. In the partner laboratories, a considerable progress has been attainedin the research of electrochemical DNA sensors as well as in analysis of proteins and other substances in recent years. This project is based on the existing results and it is oriented towards advanced studies of the properties of biomacromolecules andt heir interactions with surfaces, especially electrodes, and towards research of intermolecular interactions of DNA and/or proteins important in severe disorders (cancer, Parkinson's or Alzheimer's disease). On the basis of electrochemistry, miniaturiza
Publications
Total number of publications: 228
2007
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Label-free voltammetric detection of single nucleotide mismatches recognized by MutS protein
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, year: 2007, volume: 388, edition: 1
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Laser diode (405 nm) as an excitation source in capillary electrophoresis with laser-induced fluorescence detection analysis of GFP-tagged proteins
CECE 2007 4th international interdisciplinary meeting on bioanalysis, year: 2007
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Microanalysis of some purine derivatives by electrochemical stripping techniques
Sborník příspěvků VII. Pracovního setkání fyzikálních chemiků a elektrochemiků, year: 2007
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mRNA isolation by using paramagnetic particles and its electrochemical quantification as a tool for transcriptome analysis
Genetic toxicology and cancer prevention, year: 2007
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Resolution of overlapped reduction signals in short hetero-oligonucleotides by elimination voltammetry
Electroanalysis, year: 2007, volume: 19, edition: 2-3
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Sensitive determination of erythrosine and other red food colorants using capillary electrophoresis with laser-induced fluorescence detection
Journal of Chromatography A, year: 2007, volume: 2007, edition: 1141
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Stanovení červených potravinářských barviv kapilární elektroforézous laserem indukovanou fluorescenční detekcí
Year: 2007, type: Conference abstract
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Studium elektrodového procesu mědi na uhlíkových elektrodách
Sborník příspěvků VII. Pracovního setkání fyzikálních chemiků a elektrochemiků, year: 2007
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Studium obsahu metalothioneinu v krevnim seru pacientu se zmenenou hladinou medi nebo cisplatiny pomoci automatizovane elektrochemicke detekce
Toxcon 2007; 12. Interdisciplinary Slovak - Czech Toxicological Conference, year: 2007
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Study of redox processes on polymeric modified screen printed electrode using elimination voltammetry
Sborník příspěvků VII. Pracovního setkání fyzikálních chemiků a elektrochemiků, year: 2007