Project information
Proteins in metabolism and interaction of organisms with the environment
- Project Identification
- MSM0021622413
- Project Period
- 1/2005 - 12/2011
- Investor / Pogramme / Project type
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Ministry of Education, Youth and Sports of the CR
- Research Intents
- MU Faculty or unit
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Faculty of Science
- prof. RNDr. Jaroslav Koča, DrSc.
- Other MU Faculty/Unit
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Central European Institute of Technology
- Keywords
- proteins, structure function relationships, protein complexes, proteome, metabolome
The proposal is the follow up of the previous, in our view successful, long-term research plan "Structure-Function Relationships of Biomolecules and their Role in Metabolism" studied in 1998-2004, and partly the project "Laboratory of Structure and Dynamics of Biomolecules" in the framework of program "250" studied in 1996-2000. The results of these projects were published in tens of scientific articles communicated in renowned international journals with high impact factors. The key aim was identification and characterization of low molecular weight substances involved in metabolism of bacteria and fungi with the special attention devoted to biochemical activities and the structure.
This new proposal will deal with other proteins from various organisms, for example animal proteins binding pheromones or proteins interacting with sacharides. The function and regulation of enzymes will be studied by novel methods suitable for monitoring of in vivo activity via monitoring of intracellular metabolites. We will continue to use structural methods. Keywords of the long-term research plan are protein, its function, structure, structure-function relationships and complexes of proteins. More general keywords are proteome and metabolome. The project will focus on such hot topics of current life sciences like protein/DNA interactions, synthesis of proteins on ribosome or applications of photo-labile groups for directed geometrical changes of protein-substrate complexes and regulation of enzymatic reactions.
Publications
Total number of publications: 835
2010
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Engineering of Enzymes by Modification of Access Tunnels
Year: 2010, type:
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Extractions of isoquinoline alkaloids with butanol and octanol
Fitoterapia, year: 2010, volume: 81, edition: 6
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FastGrid -- The Accelerated AutoGrid Potential Maps Generation for Molecular Docking
Computing and Informatics, year: 2010, volume: 29, edition: 6+
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Functional studies of Ralstonia solanacearum lectin RS20L
XIV. Setkání biochemiků a molekulárních biologů, year: 2010
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grid-CICADA: Efficient Explorer of Conformational Space of Biomolecular Systems
Year: 2010, type: Appeared in Conference without Proceedings
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High-affinity lectins from pathogens: the potential targets for antiadhesive drug design
Materials Structure in Chemistry, Biology, Physics and Technology, year: 2010, volume: 17, edition: 1a
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Chromate reductase activity of the Paracoccus denitrificans ferric reductase B (FerB) protein and its physiological relevance
Archives of microbiology, year: 2010, volume: 192, edition: 11
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In silico mutagenesis and docking study of RSL lectin
Year: 2010, type: Conference abstract
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In-capillary nanoreactor based on transverse diffusion of laminar flow profiles for cytochrome P450 2C9 isoform reaction with diclofenac
Book of Abstracts, 17th International Symposium on Electro- and Liquid Phase-Separation Techniques ITP 2010, year: 2010
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Induction of iodothyronine deiodinases in cultured astrocytes by purinergic agonists. Assessment by radiometric enzyme assays
Year: 2010, type: Conference abstract