Microbial etiology of infection at surgical department: a comparison of two non-invasive methods of microbial analysis
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| Year of publication | 2012 |
| Type | Conference abstract |
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| Description | Introduction The aim of our study is the comparison of two non-invasive wound-swabbing methods used for microbiological analysis in surgical practice. We compare the wound swabbing method, which was defined in our study as the gold standard, and the imprint technique using a filter paper. Method We examined 172 samples from 68 patients from October 2008 to December 2011. Wound swabbing using sterile cotton-tipped swab. The swab was transported in a test tube with transport medium according to Amies. The imprint on the Whatman, filter paper, 5.5cm in size was used. The paper was moved using sterile tweezers from the blood agar on the area under examination and back. Results We collected 25 types of microbes by the swab and by the imprint too. The most frequently cultivated microbe in the swab was Psudomonas aeruginosa – in 25 cases, compared to the most frequently cultivated Escherichia coli in 33 cases in the imprint. The most significant difference between both methods was found in the sterile cultivation, in 15 cases. The results were processed by McNemara test using contingency tables in Statistika 10 CZ programme. The statistical significance of the test was p < 0.001 which means that the hypothesis of swab being a more accurate examination method was not confirmed. Conclusion The results obtained in our study with the imprint technique bring more accurate information than the results gained with the swab. |