Bacteriocinogeny in experimental pigs treated with short-term high-dose indomethacin with or without probiotic bacteria Escherichia coli Nissle 1917

Authors

BUREŠ Jan ŠMAJS David KVĚTINA Jaroslav FŐRSTL Miroslav ŠMARDA Jan KOHOUTOVÁ Darina KUNEŠ Martin CYRANY Jiří TACHECÍ Ilja REJCHRT Stanislav LESNÁ Jiřina VOŘÍŠEK Viktor KOPÁČOVÁ Marcela

Year of publication 2011
Type Article in Periodical
Magazine / Source Wourld Journal of Gastroenterology
MU Faculty or unit

Faculty of Medicine

Citation
Doi http://dx.doi.org/10.3748/wjg.v17.i5.609
Field Microbiology, virology
Keywords Bacteriocinogeny; Escherichia coli Nissle 1917; Experimental pigs; Indomethacin
Description AIM: To evaluate bacteriocinogeny in short-term high-dose indomethacin administration with or without probiotic Escherichia coli Nissle 1917 (EcN) in experimental pigs. METHODS: Twenty-four pigs entered the study: Group A (controls), Group B (probiotics alone), Group C (indomethacin alone) and Group D (probiotics and indomethacin). EcN (3.5x1010 bacteria/d for 14 d) and/or indomethacin (15 mg/kg per day for 10 d) were administrated orally. Anal smears before and smears from the small and large intestine were taken from all animals. Bacteriocin production was determined with 6 different indicator strains. RESULTS: The general microbiota profile was rather uniform in all animals but there was a broad diversity in coliform bacteria (parallel genotypes A, B1, B2 and D found). CONCLUSION: EcN did not exert long-term liveability in the porcine intestine. All experimental pigs remained methanogenic. Indomethacin and EcN administered together might produce the worst impact on bacteriocinogeny.
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