Acinetobacter bereziniae sp nov and Acinetobacter guillouiae sp nov., to accommodate Acinetobacter genomic species 10 and 11, respectively

Warning

This publication doesn't include Faculty of Medicine. It includes Faculty of Science. Official publication website can be found on muni.cz.
Authors

NEMEC Alexandr MUSÍLEK Martin ŠEDO Ondrej DE BAERE Thierry MAIXNEROVÁ Martina VAN DER REIJDEN Tanny J. K. ZDRÁHAL Zbyněk VANEECHOUTTE Mario DIJKSHOORN Lenie

Year of publication 2010
Type Article in Periodical
Magazine / Source International Journal of Systematic and Evolutionary Microbiology
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.1099/ijs.0.013656-0
Field Microbiology, virology
Keywords HUMAN CLINICAL SPECIMENS; GENUS ACINETOBACTER; IDENTIFICATION; BAUMANNII; PCR;
Description Acinetobacter genospecies (genomic species) 10 and 11 were described by Bouvet and Grimont in 1986 on the basis of DNA DNA reassociation studies and comprehensive phenotypic analysis. In the present study, the names Acinetobacter bereziniae sp. nov. and Acinetobacter guillouiae sp. nov., respectively, are proposed for these genomic species based on the congruence of results of polyphasic analysis of 33 strains (16 and 17 strains of genomic species 10 and 11, respectively). All strains were investigated by selective restriction fragment amplification (i.e. AFLP) analysis rpoB sequence analysis, amplified rDNA restriction analysis and tDNA intergenic length polymorphism analysis, and their nutritional and physiological properties were determined. Subsets of the strains were studied by 16S rRNA gene sequence analysis and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS or had been classified previously by DNA DNA reassociation. Results indicate that A. bereziniae and A. guillouiae represent two phenetically and phylogenetically distinct groups within the genus Acinetobacter. Based on the comparative analysis of housekeeping genes (16S rRNA and rpoB genes), these species together represent a monophyletic branch within the genus. Despite their overall phenotypic similarity, the ability to oxidize o-glucose and to grow at 38 C can be used in the presumptive differentiation of these two species from each other: with the exception of three strains that were positive for only one test, A. bereziniae strains were positive for both tests, whereas A. guillouiae strains were negative in these tests. The strains of A. bereziniae originated mainly from human clinical specimens, whereas A. guillouiae strains were isolated from different environmental sources in addition to human specimens. The type strain of A. bereziniae sp. nov. is LMG 1003(T) (=CIP 70.12(T) =ATCC 17924(T)) and that of A. guillouiae sp. nov. is LMG 988(T) (=CIP 63.46(T) =ATCC 11171(T) = CC U G 2491(T)).
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info