SINGLESTRAND CONFORMATION POLYMORPHISM OF OLIGONUCLEOTIDES IN THEIR ISOLATION BY MEANS OF MAGNETIC MICROPARTICLES

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Authors	TRNKOVÁ Libuše FOJTÍKOVÁ Martina BARTOŠKOVÁ Jana HOLUBOVÁ Sylvie KIZEK René
Year of publication	2010
Type	Article in Proceedings
Conference	X. pracovní setkání fyzikálních chemiků a elektrochemiků - sborník příspěvků
MU Faculty or unit	Faculty of Science
Citation
Field	Physical chemistry and theoretical chemistry
Keywords	conformation polymorphism;oligonucleotides; magnetic particles
Description	Studies of biomolecules depend strongly on efficiency of their isolation from biological materials. Using paramagnetic particles (MPs) represent new method for these purposes. This method, faster and less laborious than commonly used phenol chloroform extraction, is based on hybridization of target molecules on a surface of particles. For the isolation of nucleic acids MPs are modified by certain sequences of thymine or other nucleotides. Using MPs we studied the isolation of ODNs with different sequences of adenine and cytosine and ODNs with different chain lengths. It was found that singlestrand conformation polymorphism (SSCP) of ODNs is crucial phenomena for their isolation.
Related projects:	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL) Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine