Genotyping of selected potato cultivars based on structural variation of telomere-associated chromosome regions

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Authors

FAJKUS Jiří SÝKOROVÁ Eva NEPLECHOVÁ Kamila DĚDIČ Petr ŠEFČÍKOVÁ Marcela PTÁČEK Jiří

Year of publication 2007
Type Article in Periodical
Magazine / Source Vědecké práce VÚB
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords PCR; telomere-associated sequences; potato cultivars
Description Molecular genotyping techniques are of still increasing importance in agriculture practise and trade. Here we summarize results of research projects in which a novel assay for genotyping of potato cultivars was established. The assay is based on PCR using combinations of primers derived from telomeric and telomere-associated sequences. A high degree of sequence polymorphism and, namely, the variability in mutual arrangement of otherwise conserved sequence-building-blocks of subtelomeres, enabled us to establish a simple and reliable PCR-based assay to distinguish potato cultivars in 2-3 PCR reactions.
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