Effect of UV irradiation on chromosome and chromatin of HT-29 cells

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Authors

STRAŠÁK Luděk BÁRTOVÁ Eva FOJT Lukáš KOZUBEK Stanislav VETTERL Vladimír

Year of publication 2007
Type Article in Proceedings
Conference BES 2007 Toulouse, Book of Abstracts
MU Faculty or unit

Faculty of Science

Citation
Field Biophysics
Keywords UV irradiation; HT-29 cells
Description We used HT-29 cells to study effects of UV irradiation on cell level. The effect on cytoskeleton and high-ordered chromatin structure were analyzed after 48 hours of UV exposure. The cells were cultivated at 37 C in humidified atmosphere containing 5% CO2. We used FISH (fluorescence in-situ hybridization) methodology to quantify UV irradiation effect. We estimated F-actin by FITC-conjugated Phalloidin and mitochondria by MitoTracker. Images of cytoskeleton and genetic loci were acquired using confocal microscopy and analysis was performed by FISH 2.0 software. Slight morphological changes of F-actin filaments and mitochondria were observed in affected cells and nuclear condensation was found. We found changes in affected cells.
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