Surface-enhanced laser desorption-ionization/time-of-flight mass spectrometry reveals significant artifacts in serum obtained from clot activator-containing collection devices

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Authors	PILNÝ Radomír BOUCHAL Pavel BOŘILOVÁ Šárka ČEŠKOVÁ Pavla ŽALOUDÍK Jan VYZULA Rostislav VOJTĚŠEK Bořivoj VALÍK Dalibor
Year of publication	2006
Type	Article in Periodical
Magazine / Source	Clinical Chemistry
MU Faculty or unit	Faculty of Informatics
Citation
Web	Publikace ve formátu pdf
Field	Biochemistry
Keywords	proteomics; SELDI; MALDI; TOF; preanalytical variables; separator gel; clotting activator; blood collection
Description	Mass spectrometric protein profiling and pattern analysis approaches provide exciting opportunities in disease diagnosis and monitoring. Rapid advent of mass spectrometric protein profiling in recent years utilized mostly MALDI/TOF and/or SELDI/TOF platforms to analyze readily available biological materials such as serum or plasma. In our work, we studied changes in protein profiles of specimens taken in routinely used blood collecting media to see whether the newly introduced clot activator and gel-containing tubes may distort the mass spectrometric protein spectra when compared to plain sample collection tubes used previously. Here, we present an extension of previous observation and delineate a blood processing algorithm that will be applicable for analysis and banking of specimens for MALDI/SELDI-based studies in cancer patients to assess only the changes being as closely as possible of biological origin. We evaluated specific differences between groups of collection tubes Microvette Sarstedt type Neutral defined as “white” containing no gel and/or clot activator versus Microvette Sarstedt Serum, Gel Clotting activator denoted “brown” containing both. Altogether, 34 peak clusters were generated from within the mass range analyzed (3000 – 20000 m/z). In the “brown” group we detected two peaks with m/z 3957.3 and 4283.6 displaying grossly increased peak intensities whose average normalized peak areas were more than 40-fold higher comparing with the “white” groups. Remaining peaks did not display any statistical significance with respect to their occurrence in white and/or brown collection tubes, respectively.