Pilotní studie identifikace prognostických markerů u pacientů s kolorektálním karcinomem analýzou profilů genové exprese.
| Title in English | Identification of colorectal cancer prognostic markers by gene expression profiles analysis: pilot study |
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| Authors | |
| Year of publication | 2006 |
| Type | Article in Periodical |
| Magazine / Source | Klinická onkologie |
| MU Faculty or unit | |
| Citation | |
| web | http://www.linkos.cz/vzdelavani/ko_zobraz.php?i=2&ID=33&id[]=693&Submit=Zobrazit |
| Field | Oncology and hematology |
| Keywords | olorectal cancer; DNA microarray technology; gene expression profiles; pathogenesis; prognosis |
| Description | Colorectal cancer (CRC) is one of the most common malignancies. Unfortunately a significant proportion of surgically cured patients in the early stage of the disease develop progression and die from the disease. Twelve patients who had histologically confirmed left-sided colon adenocarcinoma with a volume fraction showing at least 70% of malignant tumor cells were included. Only stage II-III patients according to IUCC with no prior chemotherapy or radiotherapy were eligible for the study. Six patients were poor prognosis cases with disease free survival (DFS) lower then 36 month and six were good prognosis cases with DFS>36 month. Relative gene expression levels of 128 genes potentially involved in cancer progression and dissemination were obtained by low-density oligonucleotide microarrays (SuperArray Bioscience Corp., Bethesda, MD) from 12 primary colon cancer samples. Gene expression data analysis based on the SAM and t-test (p = 0,01) methods identified 10 genes with significantly different expression in primary tumors of patients with poor prognosis. Our preliminary data suggest that oligonucleotide microarray technology should contribute to a better understanding of the progression of colorectal cancer, and facilitate prediction of their metastatic potential. |