Application of elimination voltammetry to the resolution of adenine and cytosine signals in oligonucleotides .II. Hetero-oligodeoxynucleotides with different sequences of adenine and cytosine nucleotides
| Authors | |
|---|---|
| Year of publication | 2006 |
| Type | Article in Periodical |
| Magazine / Source | Electroanalysis |
| MU Faculty or unit | |
| Citation | |
| Field | Electrochemistry |
| Keywords | Reduction of adenine and cytosine in oligonucleotides; hanging mercury drop electrode (HMDE); elimination voltammetry with linear scan (EVLS); hetero-oligodeoxynucleotides |
| Description | Elimination voltammetry with linear scan (EVLS) was applied to the resolution of reduction signals of adenine (A) and cytosine (C) residues in short synthetic hetero-oligodeoxynucleotides (ODNs) with different sequences of A and C. The EVLS evaluation required linear sweep voltammograms measured on a hanging mercury drop electrode (HMDE) at different scan rates. Compared to linear sweep voltammetry (LSV) and usual voltammetric methods the EVLS is capable of resolving the overlapped A and C signals, specifically by using the elimination function which eliminates the charging and kinetic currents and conserves the diffusion current. Since for an adsorbed electroactive substance this elimination function yields a well readable peak-counterpeak signal, the adsorptive stripping (AdS) procedure was favorably used. While on LSV curves the only one reduction peak of A and C residues was observed in all ODNs, the EVLS yielded two separated peaks in dependence on A, C sequences and pH. Our results showed that the EVLS in connection with the AdS procedure is a useful tool for qualitative and quantitative studies of short ODNs and a promising sensitive method for the development of electrochemical sensor following the ODN sequences. |
| Related projects: |