Clustering of genes with similar regulation patterns during monocyte and granulocyte differentiation of HL-60 cells
| Authors | |
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| Year of publication | 2004 |
| Type | Article in Proceedings |
| Conference | Biophysics of the Genome |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | microarrayc |
| Description | In the present study, a high density-microarrays of human cDNA containing 19,000 genetic elements were used to search for differences in gene expression of human promyelic leukaemia cell line HL-60 during monocyte (MD) and granulocyte differentiation (GD). For differentiation to monocytes (granulocytes), the TPA, 12-O-tetradecanoylphorbol-13-acetate (DMSO) were used, respectively. A total number of 4625 (4760) genes were found to be regulated during MD (GD). The genes have been divided into groups according to their kinetics of regulation (R-groups). The genes of the same group are not distributed randomly throughout the genome but form clusters on chromosomes (R-clusters). Thus, functionally interconnected genes are frequently localized near to each other on the DNA molecule. In addition, genes of R-groups of MD (GD), if projected to GD (MD) happen to be mostly found in one or several R-groups of the other type, although the kinetics of regulation may be different (e.g. group 9 of GD and group 12 of MD). However, in most cases similar patterns of regulation correspond to each other (15, 16 of GD correspond to 3, 4 of MD). |
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