Polymorphisms in some proinflammatory genes (TNF alpha and beta, IL-1 beta, IL-6, ADAM17) in severe chronic venous disease
| Authors | |
|---|---|
| Year of publication | 2023 |
| Type | Article in Periodical |
| Magazine / Source | JEADV - journal of the European Academy of Dermatology and Venereology |
| MU Faculty or unit | |
| Citation | |
| Web | https://onlinelibrary.wiley.com/doi/10.1111/jdv.18770 |
| Doi | http://dx.doi.org/10.1111/jdv.18770 |
| Keywords | polymorphisms; proinflammatory genes; severe chronic venous disease |
| Description | BackgroundChronic venous disease (CVD) is a common disorder of lower extremities. ObjectivesThe study was scheduled to investigate the relationship between polymorphisms in major proinflammatory genes TNF alpha (-238 A/G; -308 A/G), TNF beta (NcoI), IL-1 beta (+3953 T/C); IL-6 (-174 G/C; -596 G/C) and ADAM17 (3 ' TACE) and CVD risk. Genotype-phenotype study was calculated to test possible association between examined genotypes and phenotypes of CVD. MethodsFinally, 150 CVD patients and 227 control subjects were enrolled to the study.Genotypes in proinflammatory gene polymorphisms were identified from isolated DNA by PCR method and restriction analysis. ResultsSignificant differences in genotype distribution/allelic frequencies in TNF beta gene, IL-1 beta gene and in ADAM17 gene polymorphisms were found between CVD women and control ones. In the genotype-phenotype study, identified genotypes were associated with arterial hypertension (ADAM17, IL-6-men), ischaemic heart disease (TNF alpha and beta genes), diabetes mellitus (ADAM17-women, TNF beta-men), age of CVD onset (TNF alpha and IL-6), ulceration (ADAM17), duration of ulceration (ADAM17), ulceration recurrence (ADAM17-women), home care necessity (TNF alpha), varices surgery (TNF alpha), erysipelas development (ADAM17-men) and tumour development (TNF alpha). ConclusionStudying of these polymorphisms associations can help us better identify patients at higher risk of developing severe CVD. |