Změny v dynamice Th1 odpovědi a eozinofilů u pacientů a alergickým eozinofilním astmatem v sezóně a mimo sezónu
| Title in English | Changes in Th1 response dynamics and eosinophils in patients with allergic eosinophilic asthma |
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| Authors | |
| Year of publication | 2022 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Aims: The predominant pathological mechanism of allergic eosinophilic asthma (AEA) is inflammation mediated by Th2 lymphocytes and eosinophils, but indirect signs of activation of Th1 response (ˇ IL-10 in serum, ˇ regulatory Tr1-lymphocytes, ^ INF-? in BAL) have been described. The aim of this study is to compare changes in peripheral blood eosinophils and in vitro Th1 response dynamics in AEA patients in season and out of season and to determine whether vitamin D, as an available adjunctive therapy, may have a positive effect on Tr1-lymphocyte induction in these patients. Material and Methods: From peripheral blood samples of 30 control subjects and 30 patients with AEA in season and out of season, the abundance and phenotype of eosinophil granulocytes were determined by flow cytometry. CD4 T-lymphocytes were isolated from PBMCs and cultured in vitro with anti-CD3, anti-CD46, IL-2 (differentiation to Th1 phenotype) and vitamin D (induction of a switch to Tr1-lymphocytes). RESULTS: Increased representation of eosinophil counts in whole blood was measured in in-season patients when compared to controls and out-of-season patients. Eosinophils of in-season patients showed a decrease in the expression of CD10, CD11b, CD15, CD45, CD62L and CD66b features. There was no difference in the ability of CD4 T-lymphocytes to differentiate into Th1 and Tr1-lymphocytes, including the response to vitamin D, between out-of-season patients and controls. The in-season patients responded differently to vitamin D: the first group of patients (A) was characterized by an increase in Tr1-lymphocyte numbers, as were the control subjects, whereas the second group (B) was unable to increase Tr1-lymphocyte numbers even after vitamin D supplementation. CONCLUSION: Eosinophils of patients in season could be younger developmental forms of eosinophils that eventually settle in peripheral tissues, due to changes in the expression of the studied traits. Group A patients respond to vitamin D by induction of Tr1 cells, so vitamin D could be used here as an adjunctive treatment, whereas in group B patients we assume a dysregulation of the switch from Th1 to Tr1 cells, which is not responsive to vitamin D. |
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