Carbon fiber on-line detector for monitoring human blood serum reductive capacity. A complex technical solution
| Authors | |
|---|---|
| Year of publication | 2018 |
| Type | Article in Periodical |
| Magazine / Source | Journal of Electroanalytical Chemistry |
| MU Faculty or unit | |
| Citation | |
| web | http://dx.doi.org/10.1016/j.jelechem.2018.02.037 |
| Doi | http://dx.doi.org/10.1016/j.jelechem.2018.02.037 |
| Keywords | Carbon fiber Microelectrode On-line sensing Amperometry Clinical sample Trolox |
| Description | The construction of a cylindrical carbon fiber on-line detector is presented, featuring a novel way of sealing the carbon fiber microelectrode, the design of an interchangeable carbon fiber microelectrode-based flow cell and its interface with data acquisition electronics. The two-electrode setup was used, where the regeneration of the carbon fiber working electrode surface between analyses was performed using an electrochemical procedure, typically -1/+1V potential cycling. The detector was incorporated into the flow injection analysis manifold and the applicability of the developed sensing platform tested for monitoring a hexacyanoferrate (II/III) redox probe and the oxidizable compounds trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), uric acid, and l-ascorbic acid, with dehydroascorbic acid as a negative control. Finally, the optimized procedure was utilized for the analysis of human blood serum reductive (antioxidant) capacity based on amperometric sensing and flow injection analysis at a flow rate of 10ul/min. Amperometric responses for blood serum samples were evaluated for standard deviation, whose average value was estimated to be ±0.3nA (n=20). The electrolytic efficiency of the detector was on the order of % units, e.g. 2% for trolox. The flow cell geometry and application of carbon fiber electrodes resulted in the absence of dead volumes and passivation phenomena, in contrast to conventional high-surface-area electrochemical cells. We suppose that the complex technical solution presented here could find a broad range of applications in the on-line monitoring of other reducing low-molecular bioactives and of the redox properties of various body fluids or clinical samples. |