Genome-wide transcriptional analysis of Drosophila larvae infected by entomopathogenic nematodes shows involvement of complement, recognition and extracellular matrix proteins

Investor logo

Warning

This publication doesn't include Faculty of Medicine. It includes Faculty of Science. Official publication website can be found on muni.cz.
Authors

AREFIN Badrul KUČEROVÁ Lucie DOBEŠ Pavel MARKUS Robert STRNAD Hynek WANG Zhi HYRŠL Pavel ŽUROVEC Michal THEOPOLD Ulrich

Year of publication 2014
Type Article in Periodical
Magazine / Source Journal of Innate Immunity
MU Faculty or unit

Faculty of Science

Citation
Web http://www.karger.com/Article/FullText/353734
Doi http://dx.doi.org/10.1159/000353734
Field Immunology
Keywords Basement membrane; Coagulation; Hemocyte; Insect immunity; Nematodes; Recognition molecule; Thioester-containing proteins
Description Heterorhabditis bacteriophora is an entomopathogenic nematode (EPN) which infects its host by accessing the hemolymph where it releases endosymbiotic bacteria of the species Photorhabdus luminescens. We performed a genome-wide transcriptional analysis of the Drosophila response to EPN infection at the time point at which the nematodes reached the hemolymph either via the cuticle or the gut and the bacteria had started to multiply. Many of the most strongly induced genes have been implicated in immune responses in other infection models. Mapping of the complete set of differentially regulated genes showed the hallmarks of a wound response, but also identified a large fraction of EPN-specific transcripts. Several genes identified by transcriptome profiling or their homologues play protective roles during nematode infections. Genes that positively contribute to controlling nematobacterial infections encode: a homolog of thioester-containing complement protein 3, a basement membrane component (glutactin), a recognition protein (GNBP-like 3) and possibly several small peptides. Of note is that several of these genes have not previously been implicated in immune responses.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info