Project information
Structural studies of protein-RNA complexes involved in RNA quality control

Information

This project doesn't include Faculty of Medicine. It includes Faculty of Science. Official project website can be found on muni.cz.

Project Identification

LA08008

Project Period

1/2008 - 12/2010

Investor / Pogramme / Project type

Ministry of Education, Youth and Sports of the CR

INGO

MU Faculty or unit

Faculty of Science

prof. Mgr. Richard Štefl, Ph.D.

Keywords

RNA quality control; misfolded RNA; RNA turnover; RNA recognition; protein-RNA complexes; structure; nuclear magnetic resonance

Cells seem to leave nothing to chance, including the quality control at all steps of information transfer. RNA maturation involves extensive processing that has a relatively high error rate. Cells have developed an RNA quality control mechanism which identifies improperly processed RNAs and subsequently degrades them. This mechanism ensures that only properly processed RNAs are engaged in protein synthesis. The exosome and Trf4 complex are the central molecular machines in this process. The Trf4 complex recognizes and polyadenylates the aberrant RNAs, whereas the exosome is responsible for their degradation. We propose here to use multidimensional NMR spectroscopy to determine the three-dimensional structure of several protein-RNA complexes that are involved in RNA quality control. The proposed project will reveal the mechanism by which the Trf4 complex identifies the aberrant RNAs and thus recruit them for the degradation machinery.

Publications

Total number of publications: 10

2011

1H, 13C, and 15N resonance assignments for the CTD-Interacting Domain of Nrd1 bound to Ser5-phosphorylated CTD of RNA Polymerase II

KUBÍČEK Karel PASULKA Josef ČERNÁ Hana LÖHR Frank ŠTEFL Richard

Article in Periodical

Biomolecular NMR Assignments, year: 2011, volume: 5, edition: 2, DOI
Recognition of transcription termination signal by the nuclear polyadenylated RNA-binding (Nab)3 protein

HÓBOR Fruzsina PERGOLI Roberto KUBÍČEK Karel HROŠŠOVÁ Dominika BAČÍKOVÁ Veronika ZIMMERMANN Michal PASULKA Josef HOFR Ctirad VAŇÁČOVÁ Štěpánka ŠTEFL Richard

Article in Periodical

The Journal of Biological Chemistry, year: 2011, volume: 286, edition: 5, DOI

2010

(1)H, (13)C, and (15)N chemical shift assignments for the RNA recognition motif of Nab3.

PERGOLI Roberto KUBÍČEK Karel HÓBOR Fruzsina PASULKA Josef ŠTEFL Richard

Article in Periodical

Biomolecular NMR Assignments, year: 2010, volume: 4, edition: 1, DOI
1H, 13C, and 15N chemical shift assignments of ZCCHC9

SAŇUDO ARCE Maria JACKO Martin RAMMELT Christiane VAŇÁČOVÁ Štěpánka ŠTEFL Richard

Article in Periodical

Biomolecular NMR Assignments, year: 2010, volume: 5, edition: 1, DOI
The computational view inside the dynamical behavior of the RNA-binding motive

PASULKA Josef KOČA Jaroslav ŠTEFL Richard

Conference abstract

Year: 2010, type: Conference abstract
The Solution Structure of the ADAR2 dsRBM-RNA Complex Reveals a Sequence-Specific Readout of the Minor Groove

ŠTEFL Richard OBERSTRASS Florian HOOD Jeniffer JOURDAN Muriel ZIMMERMANN Michal SKŘÍŠOVSKÁ Lenka MARIS Christophe PENG Li HOFR Ctirad EMESON Ronald ALLAIN Frederic

Article in Periodical

CELL, year: 2010, volume: 143, edition: 2

2009

Recognition of phosphorylated CTD of RNA Pol II by Nrd1

ČERNÁ Hana KUBÍČEK Karel ŠTEFL Richard

Conference abstract

Year: 2009, type: Conference abstract
Structure and RNA binding of Nab3

HÓBOR Fruzsina KUBÍČEK Karel PASULKA Josef ŠTEFL Richard

Conference abstract

Year: 2009, type: Conference abstract
The molecular dynamics study of the RNA-binding domain of ADAR2 bound to dsRNA

PASULKA Josef KOČA Jaroslav ŠTEFL Richard

Conference abstract

Year: 2009, type: Conference abstract
Transcription termination of nonpolyadenylated transcripts

HÓBOR Fruzsina ČERNÁ Hana BAČÍKOVÁ Veronika PASULKA Josef KUBÍČEK Karel ŠTEFL Richard

Conference abstract

Year: 2009, type: Conference abstract