Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering

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Authors

PROKOP Zbyněk SATO Yukari BREZOVSKÝ Jan MOZGA Tomáš CHALOUPKOVÁ Radka KOUDELÁKOVÁ Táňa JEŘÁBEK Petr ŠTĚPÁNKOVÁ Veronika NATSUME Rio LEEWEN J.G.E. JANSSEN D,B. FLORIAN J. NAGATA Yuji SENDA T. DAMBORSKÝ Jiří

Year of publication 2010
Type Article in Periodical
Magazine / Source Angewandte Chemie International Edition
MU Faculty or unit

Faculty of Science

Citation
Web http://loschmidt.chemi.muni.cz/peg/pdf/angewandte2010.pdf
Field Biochemistry
Keywords Enantioselectivity; Haloalkane Dehalogenases; protein engineering
Description Engineering of the surface loop in haloalkane dehalogenases affects their enantiodiscrimination behavior. The temperature dependence of the enantioselectivity (lnE versus 1/T) of -bromoalkanes by haloalkane dehalogenases is reversed (red data points) by deletion of the surface loop; the selectivity switches back when an additional single-point mutation is made. This behavior is not observed for -bromoesters.
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