Functional analysis of p53 tumor suppressor in yeast
| Title in English | Funcitonal analysis of p53 tumor suppressor in yeast |
|---|---|
| Authors | |
| Year of publication | 2005 |
| Type | Article in Periodical |
| Magazine / Source | Differentiation |
| MU Faculty or unit | |
| Citation | |
| Field | Oncology and hematology |
| Keywords | tumor suppressor; p53 mutation; p53 activity; FASAY |
| Description | The p53 tumor suppressor protein is a transcription factor that mediates the cells response to various kinds of stress by preventing cell division and/or inducing apoptosis. p53 gene mutations have been detected in nearly 50% of human cancers. Several approaches have been developed for detection and analyses of p53 mutations: immunochemical methods have been developed to detect p53 protein levels; molecular analyses targeting changes in DNA structure are utilized; and functional assays are used to explore the biological properties of the p53 protein. Functional analysis of separated alleles in yeast targets the transactivation capability of the p53 protein expressed in yeast cells. This method uses p53 mRNA isolated from cells and tissues to produce p53 product by RT-PCR. This method serves as a powerful tool for distinguishing various functional types of p53 mutations. Understanding of exact impact of p53 mutation on its function is an important prerequisite for establishment of efficient anticancer therapies. |
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