The City of Brno Award 2020
Ivan Rektor was one of the prize winners in The City of Brno Award in the field of medical sciences and pharmacy for 2020.
Methods available for collaborative research
First Department of Neurology, St. Anne´s University Hospital
- Invasive EEG (stereo EEG), sampling frequency 25 kHz
- HD-EEG 128-channel system
- rTMS with noninvasive neuronavigation
Contact: Milan Brázdil, mbrazd@med.muni.cz
Multimodal and Functional Imaging Laboratory - MAFIL, CEITEC, Masaryk University
- Two human whole-body MR scanners with efficient gradient system (up to 80 mT/m, 200 T/m/s) equipped with various coils, one scanner with multinuclear support (Phosphor/Hydrogen head and flexible coils available), open system for sequence development and installation of user sequences
- Equipment for fMRI stimulation and response - LCD screens, buttons, tablet, eye tracking system, physiological recordings (ECG, breathing, skin conductance, ...)
- High density EEG system with 256 channels, MR compatible, possibility of tDCS/tACS stimulation
- EEG with 32 channels, MR compatible, rTMS compatible
- rTMS system with frameless stereotactic navigation (noninvasive neuronavigation based on MR images)
- shielded electrophysiological labs with equipment for stimulation, possibility to combine TMS, EEG, physiological recording and eye-tracking
Contact: Michal Mikl, michal.mikl@ceitec.muni.cz, mafil@ceitec.muni.cz
More information at: http://mafil.ceitec.cz/en/
First Department of Pathology, St. Anne´s University Hospital
- Histopathology, standard and special staining methods
- Immunohistochemistry and immunofluorescence
- Fluorescent in situ hybridization
Contact: Marketa Hermanová, marketa.hermanova@fnusa.cz
Department of Neurology, University Hospital Brno
- Contact Heat Evoked Potentials
- Quantitative Sensory Testing (utilizing DFNS protocol)
- Skin biopsy (intraepidermal nerve fiber density)
- Corneal Confocal Microscopy (corneal nerve fibre density).
Contact: Josef Bednařík, bednarik.josef@fnbrno.cz
Department of Physiology, Faculty of Medicine, Masaryk University
- Whole cell patch clamp
- Multielectrode array
Contact: Markéta Bébarová, mbebar@med.muni.cz
Central animal facility of the Faculty of Medicine
- Operant IV self-administration of addictive substances
- Alcohol drinking model
- Behavioral testing in mice and rats (locomotion, social interactions, memory, motor impairment, etc.)
Contact: Jana Ruda-Kucerova, jkucer@med.muni.cz
Department of Anatomy, Research Group: Cellular and Molecular Neurobiology, Faculty of Medicine, Masaryk University
- Experimental models of neuropathic pain based on spinal or trigeminal nerve injury
- Behavioural tests: mechanical and thermal hypersensitivity
- In situ proteomics and image analysis
- Ex vivo electrophysiological measurement of skin-nerve preparation
Contact: Marek Joukal, mjoukal@med.muni.cz; Petr Dubový, pdubovy@med.muni.cz
More information at: www.anatomy.med.muni.cz/vyzkum/vyzkumna-skupina-bunecne-a-molekularni-neurobiologie
Department of Biomedical Engineering, International Clinical Research Center - St. Anne's University Hospital & Institute of Scientific Instruments, The Czech Academy of Sciences, Brno
- High-frequency and high-dynamic signal acquisition
- Shielded room - Faraday cages
- Automated signal processing
- Machine learning and AI utilization
- Archiving and organization of extensive data
Contact: Pavel Jurák, jurak@isibrno.cz & Jan Cimbálník, jan.cimbalnik@fnusa.cz
Institute of Scientific Instruments of the Czech Academy of Science
- Magnetic resonance 9.4T / 30cm
- Objects: in vivo mouse/rat/rabbit (anesthesized, monitored), ex vivo organs, in vitro samples
- Techniques: anatomic T1w, T2w, T2*w imaging, perfusion (DCE, DSC, ASL), diffusion (DW-MRI, DTI, DKI), relaxometry (T1, T2, T2*), spectroscopy (single-voxel PRESS/STEAM); in-magnet focused ultrasound
- Nuclei: 1H, 13C, 19F, 23Na, 31P
- Support for: experiment design, animal-use project preparation
- Animal housing
Contact: Zenon Starčuk jr., starcuk@isibrno.cz
Department of Histology and Embryology, Faculty of Medicine, Masaryk University
- Generation of human induced pluripotent stem cell lines for disease modelling
- shRNA approach for gene downregulation, vector design and cloning
- Generation of CRISPR/Cas9 knockouts
- Production of lentiviral particles
- Retinal organoids derived from human induced pluripotent stem cells
- Generation of vectors for manipulation with microRNA expression
Contact: Tomáš Bárta, tbarta@med.muni.cz
More information at: https://www.med.muni.cz/histology/tomas-barta/
- Modeling neuronal development and disease in vitro using human induced pluripotent stem cells
- iPSC generation from patients' PBMCs
- differentiation towards neural stem cells, mature neurons and 3D cerebral organoids
- CRISPR/Cas9 Knock-in and Knock/out
Contact: Dáša Bohačiaková, bohaciakova@med.muni.cz
More information at: https://www.med.muni.cz/histology/dasa-dolezalova-bohaciakova/
