Methods available for collaborative research

• Invasive EEG (stereo EEG), sampling frequency 25 kHz
• HD-EEG 128-channel system
• rTMS with noninvasive neuronavigation

Contact: Milan Brázdil, mbrazd@med.muni.cz

• Two human whole-body MR scanners with efficient gradient system (up to 80 mT/m, 200 T/m/s) equipped with various coils, one scanner with multinuclear support (Phosphor/Hydrogen head and flexible coils available), open system for sequence development and installation of user sequences
• Equipment for fMRI stimulation and response - LCD screens, buttons, tablet, eye tracking system, physiological recordings (ECG, breathing, skin conductance, ...)
• High density EEG system with 256 channels, MR compatible, possibility of tDCS/tACS stimulation
• EEG with 32 channels, MR compatible, rTMS compatible
• rTMS system with frameless stereotactic navigation (noninvasive neuronavigation based on MR images)
• shielded electrophysiological labs with equipment for stimulation, possibility to combine TMS, EEG, physiological recording and eye-tracking

Contact: Michal Mikl, michal.mikl@ceitec.muni.cz, mafil@ceitec.muni.cz

More information at: http://mafil.ceitec.cz/en/

• Histopathology, standard and special staining methods
• Immunohistochemistry and immunofluorescence
• Fluorescent in situ hybridization

 Contact: Marketa Hermanová, marketa.hermanova@fnusa.cz

• Contact Heat Evoked Potentials
• Quantitative Sensory Testing (utilizing DFNS protocol)
• Skin biopsy (intraepidermal nerve fiber density)
• Corneal Confocal Microscopy (corneal nerve fibre density).

Contact: Josef Bednařík, bednarik.josef@fnbrno.cz

• Whole cell patch clamp
• Multielectrode array 

Contact: Markéta Bébarová, mbebar@med.muni.cz

• Operant IV self-administration of addictive substances
• Alcohol drinking model
• Behavioral testing in mice and rats (locomotion, social interactions, memory, motor impairment, etc.)

Contact: Jana Ruda-Kucerova, jkucer@med.muni.cz

• Experimental models of neuropathic pain based on spinal or trigeminal nerve injury
• Behavioural tests: mechanical and thermal hypersensitivity
• In situ proteomics and image analysis
• Ex vivo electrophysiological measurement of skin-nerve preparation

Contact: Marek Joukal, mjoukal@med.muni.cz; Petr Dubový, pdubovy@med.muni.cz

More information at: www.anatomy.med.muni.cz/vyzkum/vyzkumna-skupina-bunecne-a-molekularni-neurobiologie

• High-frequency and high-dynamic signal acquisition
• Shielded room - Faraday cages
• Automated signal processing
• Machine learning and AI utilization
• Archiving and organization of extensive data

Contact: Pavel Jurák, jurak@isibrno.cz & Jan Cimbálník, jan.cimbalnik@fnusa.cz

• Magnetic resonance 9.4T / 30cm
• Objects: in vivo mouse/rat/rabbit (anesthesized, monitored), ex vivo organs, in vitro samples
• Techniques: anatomic T1w, T2w, T2*w imaging, perfusion (DCE, DSC, ASL), diffusion (DW-MRI, DTI, DKI), relaxometry (T1, T2, T2*), spectroscopy (single-voxel PRESS/STEAM); in-magnet focused ultrasound
• Nuclei: 1H, 13C, 19F, 23Na, 31P
• Support for: experiment design, animal-use project preparation
• Animal housing

Contact: Zenon Starčuk jr., starcuk@isibrno.cz

• Generation of human induced pluripotent stem cell lines for disease modelling
• shRNA approach for gene downregulation, vector design and cloning
• Generation of CRISPR/Cas9 knockouts
• Production of lentiviral particles
• Retinal organoids derived from human induced pluripotent stem cells
• Generation of vectors for manipulation with microRNA expression

Contact: Tomáš Bárta, tbarta@med.muni.cz

More information at: https://www.med.muni.cz/histology/tomas-barta/

• Modeling neuronal development and disease in vitro using human induced pluripotent stem cells
• iPSC generation from patients' PBMCs
• differentiation towards neural stem cells, mature neurons and 3D cerebral organoids
• CRISPR/Cas9 Knock-in and Knock/out

Contact: Dáša Bohačiaková, bohaciakova@med.muni.cz

More information at: https://www.med.muni.cz/histology/dasa-dolezalova-bohaciakova/