Methods available for collaborative research
First Department of Neurology, St. Anne´s University Hospital
- Invasive EEG (stereo EEG), sampling frequency 25 kHz
- HD-EEG 128-channel system
- rTMS with noninvasive neuronavigation
Contact: Milan Brázdil, mbrazd@med.muni.cz
Multimodal and Functional Imaging Laboratory - MAFIL, CEITEC, Masaryk University
- Two human whole-body MR scanners with efficient gradient system (up to 80 mT/m, 200 T/m/s) equipped with various coils, one scanner with multinuclear support (Phosphor/Hydrogen head and flexible coils available), open system for sequence development and installation of user sequences
- Equipment for fMRI stimulation and response - LCD screens, buttons, tablet, eye tracking system, physiological recordings (ECG, breathing, skin conductance, ...)
- High density EEG system with 256 channels, MR compatible, possibility of tDCS/tACS stimulation
- EEG with 32 channels, MR compatible, rTMS compatible
- rTMS system with frameless stereotactic navigation (noninvasive neuronavigation based on MR images)
- shielded electrophysiological labs with equipment for stimulation, possibility to combine TMS, EEG, physiological recording and eye-tracking
Contact: Michal Mikl, michal.mikl@ceitec.muni.cz, mafil@ceitec.muni.cz
More information at: http://mafil.ceitec.cz/en/
First Department of Pathology, St. Anne´s University Hospital
- Histopathology, standard and special staining methods
- Immunohistochemistry and immunofluorescence
- Fluorescent in situ hybridization
Contact: Marketa Hermanová, marketa.hermanova@fnusa.cz
Department of Neurology, University Hospital Brno
- Contact Heat Evoked Potentials
- Quantitative Sensory Testing (utilizing DFNS protocol)
- Skin biopsy (intraepidermal nerve fiber density)
- Corneal Confocal Microscopy (corneal nerve fibre density).
Contact: Josef Bednařík, bednarik.josef@fnbrno.cz
Department of Physiology, Faculty of Medicine, Masaryk University
- Whole cell patch clamp
- Multielectrode array
Contact: Markéta Bébarová, mbebar@med.muni.cz
Central animal facility of the Faculty of Medicine
- Operant IV self-administration of addictive substances
- Alcohol drinking model
- Behavioral testing in mice and rats (locomotion, social interactions, memory, motor impairment, etc.)
Contact: Jana Ruda-Kucerova, jkucer@med.muni.cz
Department of Anatomy, Research Group: Cellular and Molecular Neurobiology, Faculty of Medicine, Masaryk University
- Experimental models of neuropathic pain based on spinal or trigeminal nerve injury
- Behavioural tests: mechanical and thermal hypersensitivity
- In situ proteomics and image analysis
- Ex vivo electrophysiological measurement of skin-nerve preparation
Contact: Marek Joukal, mjoukal@med.muni.cz; Petr Dubový, pdubovy@med.muni.cz
More information at: www.anatomy.med.muni.cz/vyzkum/vyzkumna-skupina-bunecne-a-molekularni-neurobiologie
Department of Biomedical Engineering, International Clinical Research Center - St. Anne's University Hospital & Institute of Scientific Instruments, The Czech Academy of Sciences, Brno
- High-frequency and high-dynamic signal acquisition
- Shielded room - Faraday cages
- Automated signal processing
- Machine learning and AI utilization
- Archiving and organization of extensive data
Contact: Pavel Jurák, jurak@isibrno.cz & Jan Cimbálník, jan.cimbalnik@fnusa.cz
Institute of Scientific Instruments of the Czech Academy of Science
- Magnetic resonance 9.4T / 30cm
- Objects: in vivo mouse/rat/rabbit (anesthesized, monitored), ex vivo organs, in vitro samples
- Techniques: anatomic T1w, T2w, T2*w imaging, perfusion (DCE, DSC, ASL), diffusion (DW-MRI, DTI, DKI), relaxometry (T1, T2, T2*), spectroscopy (single-voxel PRESS/STEAM); in-magnet focused ultrasound
- Nuclei: 1H, 13C, 19F, 23Na, 31P
- Support for: experiment design, animal-use project preparation
- Animal housing
Contact: Zenon Starčuk jr., starcuk@isibrno.cz
Department of Histology and Embryology, Faculty of Medicine, Masaryk University
- Generation of human induced pluripotent stem cell lines for disease modelling
- shRNA approach for gene downregulation, vector design and cloning
- Generation of CRISPR/Cas9 knockouts
- Production of lentiviral particles
- Retinal organoids derived from human induced pluripotent stem cells
- Generation of vectors for manipulation with microRNA expression
Contact: Tomáš Bárta, tbarta@med.muni.cz
More information at: https://www.med.muni.cz/histology/tomas-barta/
- Modeling neuronal development and disease in vitro using human induced pluripotent stem cells
- iPSC generation from patients' PBMCs
- differentiation towards neural stem cells, mature neurons and 3D cerebral organoids
- CRISPR/Cas9 Knock-in and Knock/out
Contact: Dáša Bohačiaková, bohaciakova@med.muni.cz
More information at: https://www.med.muni.cz/histology/dasa-dolezalova-bohaciakova/
