Apoptosis in pig leucocytes during cardiopulmonary bypass

Varování

Publikace nespadá pod Lékařskou fakultu, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.

Autoři	MATALOVÁ Eva ŠPANOVÁ Alena RITTICH Bohuslav WAGNER Robert KOVÁŘŮ František
Rok publikování	1999
Druh	Článek ve sborníku
Konference	Inflammatory Paradigms and the Vasculature
Fakulta / Pracoviště MU	Přírodovědecká fakulta
Citace
Obor	Genetika a molekulární biologie
Popis	The aim of this study was to observe programmed cell death (PCD) activation in pig leucocytes durind cardiopulmonary bypass (CPB). Blood samples were taken prior to, during, and after CPB. Apoptosis was detected using TUNEL assay and by typical apoptotic morphology changes (Giemsa staining). Apoptotic DNA fragmentation was proved in high percentage of cells in the operating beginning. A decrease in the number of apoptotic cells was detected in the surviving pigs after the aorta clip release. On the other hand, the number of apoptotic leucocytes did not significantly change in pigs died owing to postperfusion syndrom. The same alterations in percentage of apoptotic cells were proved in bone marrow after chest opening, after surgery and in dead animals. These results suggested that PCD can play a role in the postperfusion syndrome after CPB.
Související projekty:	Postperfuzní syndrom II.