Bacteriome analysis of cystic fluids from odontogenic cysts – a pilot study
| Autoři | |
|---|---|
| Rok publikování | 2023 |
| Druh | Konferenční abstrakty |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | The diagnosis of developmental odontogenic cyst (OC) is not always clear; it may show inflammatory changes, and in that case the diagnostic features can be lost. As developmental OCs can become secondarily infected, the aim of this pilot study was to investigate bacteriomes of fluids from developmental OCs (e.g. dentigerous cyst, DC; odontogenic keratocyst, OKC) with/without inflammatory changes and compare them with findings in fluids from inflammatory OCs (radicular cyst, RC). Cystic fluids from 38 DCs, 12 OKCs, and 34 RCs were obtained by aspiration using a sterile syringe. Microbial DNA was isolated from cystic fluids and negative controls (N=32) using the QIAamp DNA Mini Kit; isolates were spiked with a mock community. 16S rRNA gene libraries were sequenced on Illumina MiSeq instrument with ?5000 reads per sample. A sample with a relative abundance of a bacterial genus >20% was considered “positive”. Alpha diversity was significantly decreased in fluids from OCs with inflammation (N=42) in comparison with those without inflammation (N=42; Shannon index, p<0.05). Bacteriomes of fluids from DCs without inflammatory changes were similar to negative controls (p>0.05). Interestingly, 75% of fluids from OKCs were “positive” for DNA of one or more bacterial genera (e.g. Porphyromonas, Prevotella, Parvimonas, Haemophilus). Significant difference in the representation of "positive" samples was observed between those from DC and RC (26% vs. 56% of samples, p<0.05). RC fluids were “positive” for Fusobacterium, Porphyromonas, Prevotella, Fretibacterium, Aggregatibacter, and others. In conclusion, non-sterile cystic fluids from inflammatory DCs, OKCs, and RCs contain DNA from anaerobic periodontal pathogens. These bacteria might play a role in OKCs’ development. In line with our assumption, the content of DCs without inflammatory changes appears to be sterile in most cases. |
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