Dental cell type atlas reveals new stem and differentiated cell types in mouse and human teeth
| Autoři | |
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| Rok publikování | 2019 |
| Druh | Vyžádané přednášky |
| Citace | |
| Popis | Continuously growing mouse teeth serve already for several decades as an excellent model system for studying tooth development, stem cell niche, and importantly for understanding mutually interdependent epithelial-mesenchymal interactions. Currently, we introduced a new unbiased approach on how to analyse cell developmental pathways of teeth leading to dentin and enamel formation. We deciphered how cells in continuously growing tooth gradually differentiate from the stem cells through all the intermediate stages into late mature stages. Moreover, we report previously unappreciated cellular complexity of the mouse incisor, including new spatially-restricted stem, progenitor and differentiated populations and compare this model system with the human adult, growing and carieslesioned teeth. This comparison revealed specific parallelisms in mechanisms of dental growth in humans and mice. Beyond the main mesenchymal and epithelial compartments, we demonstrate a surprising role of the immune system as a mediator of the epithelialmesenchymal interactions required for proper development. Based on our findings we designed several dozen of knockout and conditional knockout animals which will become useful models in the field of tooth development and also may lead to a better understanding of human congenital teeth malformations of hitherto unknown aetiology. Finally, based on the detailed map of gene expression patterns during the differentiation of odontoblasts and ameloblasts cell lineages, our results brought new insights into the controlling of the process leading to dentin and enamel formation which should be of utmost importance for dental reconstructive engineering. |